Journal Papers

  1. P. Pham, S. Rooholghodos, J. Choy and X. Luo, “Constructing synthetic ecosystems with biopolymer fluitrodes”, Advanced Biosystems, 2018, In press.
  2. A. Alshehri, O. C. Wilson, B. R. Dahal, J. Philip, X. Luo and C. B. Raub, “Magnetic nanoparticle-loaded alginate beads for local micro-actuation of in vitro tissue constructs”, Colloids and Surfaces B: Biointerfaces, 2017, 159, 945–955. [link]
  3. W. Shang, C. Tsao, X. Luo, M. Teodoro, R. McKay, D. Quan, H. Wu, G. F. Payne and W. E. Bentley, “A simple and reusable bilayer microfluidic device for the studies of bacterial gradient-sensing behaviors”, Biomicrofluidics, 2017, 11, 044114, 1-10. [link]
  4. K. Li, S. O. Correa, P. Pham, C. Raub and X. Luo, “Birefringence microscopy revealing crystalline gelation mechanism of biofabricated chitosan membranes in microfluidics”, Biofabrication, 2017, 9, 034101. [link]
  5. P. Pham, T. Vo and X. Luo, “Steering air bubbles with an add-on vacuum layer for biopolymer membranes biofabrication in PDMS microfluidics”, Lab on a Chip, 2017, 17, 248-255. [link]
  6. H. C. Wu, D. N. Quan, C. Y. Tsao, Y. Liu, J. L. Terrell, X. Luo, J. C. Yang, G. F. Payne and W. E. Bentley, “Conferring biological activity to native spider silk: A biofunctionalized protein-based microfiber”, Biotechnology & Bioengineering, 2017, 114, 83-95. [link]
  7. C. J. Wolfram, G. W. Rubloff and X. Luo, “Perspectives in Flow-Based Microfluidic Gradient Generators for Characterizing Bacterial Chemotaxis”, Biomicrofluidics, 2016, 10, 061301, 1-12. [link]
  8. X. Luo, T. Vo, F. Jambi, P. Pham and J. Choy, “Microfluidic partition with in situ biofabricated semipermeable biopolymer membranes for static gradient generation”, Lab on a Chip, 2016, DOI: 10.1039/C6LC00742B.
  9. X. L. Luo, C. Y. Tsao, H. C. Wu, D. N. Quan, G. F. Payne, G. W. Rubloff and W. E. Bentley, “Distal modulation of cell-cell signaling in a synthetic ecosystem in partitioned microfluidics”, Lab on a Chip, 2015, 8:1842-51.
  10. X. L. Luo, H. C. Wu, J. Betz, W. E. Bentley and G. W. Rubloff, “Air bubble-initiated biofabrication of freestanding, semi-permeable biopolymer membranes in PDMS microfluidics”, Biochemical Engineering Journal, 2014, 89, 2-9.
  11. J. F. Betz, Y. Cheng, C. Y. Tsao, A. Zargar, H. C. Wu, X. L. Luo, G. F. Payne, W. E. Bentley and G. W. Rubloff, “Optically clear alginate hydrogels for spatially controlled cell entrapment and culture at microfluidic electrode surfaces”, Lab on a Chip, 2013, 10, 1854-1858.
  12. T. Gordonov, B. Liba, J. L. Terrell, Y. Cheng, X. L. Luo, G. F. Payne and W. E. Bentley, “Bridging the Bio-Electronic Interface with Biofabrication”,  Journal of Visualized Experiments, 2012, 64, e4231, DOI: 10.3791/4231.
  13. X. L. Luo, “Biofabrication in microfluidics: a converging fabrication paradigm to exploit biology in microsystems”, Journal of Bioengineering & Biomedical Science, 2012, 2:e104.
  14. X. L. Luo, H. C. Wu, C. Y. Tsao, Y. Cheng, J. Betz, G. F. Payne, G. W. Rubloff and W. E. Bentley, “Biofabrication of stratified biofilm mimics for observation and control of bacterial signaling”, Biomaterials, 2012, 33, 5136-5143.
  15. Y. Cheng, X. L. Luo, G. F. Payne and G. W. Rubloff, “Biofabrication: Programmable Assembly of Hydrogels in Microfluidics as Scaffolds for Biology”, Journal of Materials Chemistry, 2012, 22, 7659-7666. Highlights article.
  16. J. L. Terrell1, T. Gordonov, Y. Cheng, H. C. Wu, D. Sampey, X. L. Luo, C. Y. Tsao, R. Ghodssi, G. W. Rubloff, G. F. Payne and W. E. Bentley, “Integrated Biofabrication for Electro-addressed In-Film Bioprocessing”, Biotechnology Journal, 2012, 7, 428-439.
  17. Y. Cheng, C. Y. Tsao, H. C. Wu, X. L. Luo, J. L. Terrell, J. Betz, G. F. Payne, W. Bentley and G. W. Rubloff, “Electroaddressing functionalized polysaccharides as model biofilms for interrogating cell signaling”, Advanced Functional Materials, 2012, 22, 519-528.
  18. X. L. Luo, S. B. Buckhout-White, W. E. Bentley and G. W. Rubloff, “Biofabrication of chitosan-silver composite SERS substrates enabling quantification of adenine by spectroscopic shift”, Biofabrication, 2011, 3, 034108.
  19. Y. Cheng, X. L. Luo, J. Betz, H. C. Wu, C. Y. Tsao, G. F. Payne, W. Bentley and G. W. Rubloff, “Biocompatible multi-address 3D cell assembly in microfluidic devices using spatially programmable gel formation”, Lab on a chip, 2011, 11, 2316-2318.
  20. Y. Cheng, X. L. Luo, J. Betz, G. F. Payne, W. Bentley and G. W. Rubloff, “Mechanism of anodic electrodeposition of calcium alginate”, Soft Matter, 2011, 7, 5677-5684.
  21. R. Ghodssi, P. Dykstra, M. Meyer, S. Koev, K. Gerasopoulos, X. Luo, G. Rubloff, W. Bentley, G. Payne and J. Culver, “Integration of Diverse Biological Materials in Micro/Nano Devices”, Advanced Materials and Technologies for Micro/Nano-Devices, Sensors and Actuators, NATO Science for Peace and Security Series B: Physics and Biophysics, 2010, Part 4, 275-285.
  22. R. I. Bauer, X. L. Luo and W. E. Bentley, “Customizable PDMS chamber for cell imaging”, Chips & Tips (Lab on a Chip), Sept 13, 2010.
  23. S. T. Koev, P. H. Dykstra, X. L. Luo, G. W. Rubloff, W. E. Bentley, G. F. Payne and R. Ghodssi, “Chitosan: an integrative biomaterial for lab-on-a-chip devices”, Lab on a chip, 2010, 10, 3026-3042.
  24. Y. Cheng, X. L. Luo, J. Betz, S. Buckhout-White, O. Bekdash, G. F. Payne, W. E. Bentley and G. W. Rubloff, “In situ quantitative visualization and characterization of chitosan electrodeposition with paired sidewall electrodes”, Soft Matter, 2010, 6, 3177-3183
  25. R. Fernandes, X. L. Luo (co-first authors), C. Y. Tsao, G. F. Payne, R. Ghodssi, G. W. Rubloff and W. E. Bentley, “Biological nanofactories facilitate spatially selective capture and manipulation of quorum sensing bacteria in a bioMEMS device”, Lab on a Chip, 2010, 10, pp. 1128-1134, in Chemical Technology; Journal inside cover image.
  26. X. L. Luo, D. Larios Berlin, J. Betz, G. F. Payne, W. E. Bentley and G. W. Rubloff, “In situ generation of pH gradients in microfluidic devices for biofabrication of freestanding, semi-permeable chitosan membranes”, Lab on a Chip, 2010, 10, pp. 59-65, in Chemical Technology.
  27. X. L. Luo, D. Larios Berlin, S. B. Buckhout-White, R. Ghodssi and G. W. Rubloff, “Design optimization for bioMEMS studies of enzyme-controlled metabolic pathways”, Biomedical Microdevices, 2008, 9, pp. 899-908.
  28. X. L. Luo, A. T. Lewandowski, H. M. Yi, R. Ghodssi, G. F. Payne, W. E. Bentley and G. W. Rubloff, “Programmable assembly of a metabolic pathway enzyme in a pre-packaged reusable bioMEMS device”, Lab on a Chip, 2008, 8, pp. 420-430.
  29. A. T. Lewandowski, H. M. Yi, X. L. Luo, G. F. Payne, R. Ghodssi, G. W. Rubloff and W. E. Bentley, “Protein assembly onto patterned microfabricated devices through enzymatic activation of fusion pro-tag”, Biotechnology and Bioengineering, 2008, 99, pp. 499-507.
  30. J. J. Park, X. L. Luo, H. M. Yi, T. M. Valentine, G. F. Payne, W. E. Bentley, R. Ghodssi and G. W. Rubloff, “Chitosan-mediated in situ biomolecule assembly in completely packaged microfluidic devices”, Lab on a Chip, 2006, 6, pp. 1315-1321.

Refereed Conference Proceeding

Presenting author underlined

  1. X. L. Luo and G. W. Rubloff, “In situ microfluidic partition with semi-permeable membranes for static gradient generation”, Proceedings of the 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2014), San Antonio, USA, October 26-30, 2014. (Poster presentation, late news submission)
  2. X. L. Luo, G. W. Rubloff and W. E. Bentley, “Numeric modeling of cell-cell signaling in partitioned microfluidics”, Proceedings of the 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2013), Freiburg-Black Forest, Germany, October 27-31, 2013. (Poster presentation)
  3. X. L. Luo, H. C. Wu, C. Y. Tsao, Y. Cheng, G. W. Rubloff and W. E. Bentley, “In situ biofabrication of stratified biofilm mimics for direct observation of bacterial signaling”, Proceedings of 2012 IEEE 38th Annual Northeast Bioengineering Conference (NEBEC 2012), Philadelphia, PA, USA, March 16-18, 2012, pp. 453-454. (Oral presentation)
  4. Yi Cheng, J. Terrell, X. L. Luo, H. C. Wu, C. Y. Tsao, J. Betz, W. E. Bentley and G. W. Rubloff, “Immobilization and culturing of mammalian cells with biocompatible electrodeposition of calcium alginate gel in microfluidic devices”, Proceedings of the 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2011), Seattle, WA, USA, October 2-6, 2011, pp. 121-123. (Poster presentation)
  5. X. L. Luo, H. C. Wu, C. Y. Tsao, Y. Cheng, G. W. Rubloff and W. E. Bentley, “Micro-sandwich in microfluidics: 3D biopolymer membranes for cell assembly”, Proceedings of the 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2010), Groningen, Netherlands, October 3-7, 2010, pp. 1601-1603. (Poster presentation, presented by J. Betz)
  6. Y. Cheng, X. L. Luo, J. Betz, G. F. Payne, W. E. Bentley and G. W. Rubloff, “Electroaddressable Biofabrication of Polysaccharide Scaffolds for Programmable Cell Assembly”, Proceedings of 2010 International Conference on Biofabrication, Philadelphia, PA, USA, Oct 3-6, 2010, pp. 88.  (Oral presentation)
  7. X. L. Luo, H. C. Wu, C. Y. Tsao, Y. Cheng, R. Ghodssi, G. F. Payne, G. W. Rubloff and W. E.  Bentley, “Biofabrication of 3D biopolymer scaffolds for cell assembly in microfluidics”, Proceedings of 2010 International Conference on Biofabrication, Philadelphia, PA, USA, Oct 3-6, 2010, pp. 106. (Oral presentation)
  8. Y. Cheng, X. L. Luo, J. Betz, O. Bekdash and G. W. Rubloff, “Mechanism and direct visualization of electrodeposition of the polysaccharide chitosan”, IFMBE Proceedings: 26th Southern Biomedical Engineering Conference 2010 (SBEC 2010), eds. K. E. Herold, W. E. Bentley and J. Vossoughi, Springer Berlin Heidelberg New York, 2010, vol. 32, pp. 401-403. (Oral presentation)
  9. X. L. Luo, D. Larios Berlin, S. Buckout-White, W. E. Bentley, G. F. Payne, R. Ghodssi and G. W. Rubloff, "Minimizing parasitic reactions for enzyme-controlled metabolic pathways investigated in bioMEMS”, Proceedings of the 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2008), San Diego, CA, USA, October 12-16, 2008, pp. 1039-1041. (Poster presentation)
  10. S. Buckout-White, X. L. Luo, D. Larios Berlin, G. F. Payne, W. E. Bentley, R. Ghodssi and G. W. Rubloff, "A platform for in-situ sensing of biomolecules using a chitosan mediated SERS substrate fabricated in microfluidics”, Proceedings of the 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2008), San Diego, CA, October 12-16, 2008, pp.928-930. (Poster presentation)
  11. X. L. Luo, A. T. Lewandowski, D. Berlin, G. F. Payne, R. Ghodssi, W. E. Bentley and G. W. Rubloff, “Applications and improvements of bioMEMS for chitosan-mediated enzyme assembly and catalytic activity”, Proceedings of the First Shenyang International Colloquium on Microfluidics (SICOM I), Shenyang, China, October 21-24, 2007, pp. 204-207. (Oral presentation)
  12. X. L. Luo, A. T. Lewandowski, G. F. Payne, R. Ghodssi, W. E. Bentley and G. W. Rubloff, “Enzyme assembly and catalytic activity in a reusable bioMEMS platform for metabolic engineering”, Proceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2007), Paris, France, October 7-11, 2007, pp. 1616-1618. (Poster presentation)
  13. X. L. Luo, A. T. Lewandowski, H. Yi, G. F. Payne, R. Ghodssi, W. E. Bentley and G. W. Rubloff, “Signal-guided sequential assembly of nano-bio-components in a completely packaged microfluidic environment”, Proceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2007), Paris, France, October 7-11, 2007, pp. 784-786. (Poster presentation)
  14. X. L. Luo, J. J. Park, H. Yi, A. T. Lewandowski, W. E. Bentley, G. F. Payne, R. Ghodssi and G. W. Rubloff, “Chitosan-mediated enzyme assembly toward rebuilding a metabolic pathway in the microfluidic environment”, Proceedings of Materials Research Society 2007 Spring Meeting, San Francisco, CA, April 9-13, 2007. (Oral presentation)
  15. J. J. Park, X. L. Luo, H. Yi, R. Ghodssi and G.W. Rubloff, “In situ biomolecule assembly and activity within completely packaged microfluidic devices”, Proceedings of IEEE/NLM Life Science Systems and Applications Workshop, Bethesda, MD, July 13-14, 2006, pp. 88-89. (Oral presentation)
  16. X. L. Luo, J. S. J. Chen, M. Wolfson, C. Philips and T. Shaffer, “PIV measurement of fluid flow inside a human uterus model for cryoablation”, Proceedings of 2003 IEEE 29th Annual Bioengineering Conference, Newark, NJ, March 22-23, 2003, pp. 225-226. (Oral presentation)

Conference Abstracts

Presenting author underlined

  1. T. Vo, P. Pham, J.S. Choy and X. Luo, “ Quantitative Yeast Cell Dynamics in Static Chemical Gradients”, 2016 BMES Annual Meeting, Minneapolis, MN, October 5-8, 2016. (Poster presentation, to appear)
  2. P. Pham , T. Vo and X. Luo, “Deploying Air Bubbles In Two Layer Microfluidic Devices For In Situ Fabrication Of Biopolymer Membranes”, ASME's International Mechanical Engineering Congress and Exposition (IMECE), Phoenix, AZ, November 11-17, 2016. (Accepted)
  3. T. Vo , J. Choy and X. Luo, “Microfluidic Generated Static Gradients of Biomolecules for Studying Cell Dynamics”, ASME's International Mechanical Engineering Congress and Exposition (IMECE), Phoenix, AZ, November 11-17, 2016. (Accepted)
  4. X. Luo , “Lipid Bilayer Formation in Microfluidics towards Highly Stable Model Cell Membrane”, ASME 2016 International Conference on Nanochannels, Microchannels and Minichannels (ICNMM), Washington, DC, July 10-14, 2016. (Oral presentation)
  5. X. Luo and L. Bautista, “Capillary Viscometer Based on Ideal Gas Law”, ASME 2016 International Conference on Nanochannels, Microchannels and Minichannels (ICNMM), Washington, DC, July 10-14, 2016. (Oral presentation)
  6. A. Laprade and X. Luo, “Lipid Bilayer Formation In PDMS Microfluidics Towards Highly Stable Artificial Cell Membrane”, 2015 BMES Annual Meeting , Tampa, FL, October 7-10, 2015. (Oral presentation, undergraduate research submission)
  7. X. Luo , C. Wolfram, H. Wu, W. Bentley and G. Rubloff, “Static Gradients Generated with Biofabricated Semi-permeable Biopolymer Membranes in Microfluidics for Bacterial Chemotaxis Studies”, 2015 BMES Annual Meeting, Tampa, FL, October 7-10, 2015. (Oral presentation)
  8. D. Renner, X. L. Luo, “Pressure measurement in PDMS microchannels using the ideal gas law”, 2014 BMES Annual Meeting, San Antonio, TX, October 22-25, 2014. (Poster presentation, undergraduate research submission)
  9. X. L. Luo, J. Terrell, H. C. Wu, C. Y. Tsao and W. E. Bentley, “Integrated in-film bioprocessing in microfluidics enabled with biofabricated membranes and cell-gel composites”, 2013 BMES Annual Meeting, Seattle, WA, September 25-28, 2013. (Poster presentation)
  10. X. L. Luo, H. C. Wu, C. Y. Tsao, G. W. Rubloff and W. E. Bentley, “Active interception and elimination of bacterial signaling with engineered cell communities: towards in vitro models of intestinal flora”, 2012 BMES Annual Meeting, Atlanta, GA, October 24-27, 2012. (Oral presentation)
  11. X. L. Luo, H. C. Wu, C. Y. Tsao, G. W. Rubloff and W. E. Bentley, “In-Situ Biofabrication of Spatially Programmed Biofilm Mimics for Direct Observation of Bacterial Signaling”, 2012 AIChE Annual Meeting, Pittsburg, PA, October 28-November 2, 2012. (Oral presentation)
  12. X. L. Luo, H. C. Wu, C. Y. Tsao, Y. Cheng, J. Betz, G. W. Rubloff and W. E. Bentley, “Bacterial communication in controlled microevironments”, AVS 588th International Symposium, Nashville, TN, October 30-November 4, 2011. (Oral presentation)
  13. X. L. Luo, H. C. Wu, C. Y. Tsao, Y. Cheng, J. Betz, G. W. Rubloff and W. E. Bentley, “Cell signaling in membrane-based 3D microenvironments towards deciphering quorum sensing”, 2011 Materials Research Society (MRS) Spring Meeting and Exhibit, San Francisco, CA, April 25-29, 2011.  (Oral presentation)
  14. X. L. Luo, H. C. Wu, C. Y. Tsao, Y. Cheng, G. W. Rubloff and W. E. Bentley, “Micro-sandwich in microfluidics: 3D biopolymer membranes for cell assembly”, BMES Annual Meeting 2010, Austin, TX, USA, October 6-9, 2010. (Poster presentation)
  15. X. L. Luo, G. W. Rubloff and W. E. Bentley, “Quantification of Adenine using Chitosan-mediated SERS Substrates”, the 26th Southern Biomedical engineering Conference (SBEC 2010), College Park, MD, USA, April 30-May 2, 2010. (Oral presentation)
  16. X. L. Luo, D. Larios Berlin, G. W. Rubloff and W. E. Bentley, “In situ biofabrication of stimuli responsive biopolymer membranes in Microfluidics”, Division of Biochemical Technology, American Chemistry Society Fall 2009 National Meeting, Washington DC, Aug 16-20, 2009.  (Oral presentation)
  17. X. L. Luo, D. Larios Berlin, J. Gair and G. W. Rubloff, “Biofabrication and enzymatic functionalization of free-standing chitosan membranes in microfluidic Network”, 2009 Materials Research Society (MRS) Spring Meeting and Exhibit, San Francisco, CA, April 13-17, 2009.  (Special oral presentation for Graduate Student Awards)
  18. X. L. Luo, R. Fernandes, W. E. Bentley, G. F. Payne, R. Ghodssi and G. W. Rubloff, “Spatially localized quorum sensing in a microfluidic device”, 2009 Materials Research Society (MRS) Spring Meeting and Exhibit, San Francisco, CA, April 13-17, 2009.  (Oral presentation)
  19. X. L. Luo, D. Larios Berlin, W. E. Bentley, G. F. Payne, R. Ghodssi and G. W. Rubloff, "Avoiding parasitic reactions due to interconnect dead volume and non-specific binding in microfluidics”, American Vacuum Society 54th International Symposium, Boston, MA, October 19-24, 2008. (Oral presentation)
  20. W. E. Bentley, X. L. Luo, H. Wu, S. Koev, C. Tsao, R. Fernandes, G. F. Payne, R. Ghodssi and G. W. Rubloff, "High content biofunctionalized bioMEMS for revealing quorum sensing regulation and biofilm formation”, BMES 2008 Annual Fall Meeting, St. Louis, MO, October 2-4, 2008. (Poster presentation)
  21. X. L. Luo, D. Larios Berlin, W. E. Bentley, G. F. Payne, R. Ghodssi and G. W. Rubloff, "BioMEMS study and design optimization of in vitro enzyme-controlled metabolic pathways”, BMES 2008 Annual Fall Meeting, St. Louis, MO, October 2-4, 2008. (Oral presentation)
  22. X. L. Luo, D. Larios Berlin, J. Gair and G. W. Rubloff, "Microfabrication and biofunctionalization of permeable vertical chitosan Membranes in microfluidics”, BMES 2008 Annual Fall Meeting, St. Louis, MO, October 2-4, 2008. (Poster presentation)
  23. X. L. Luo, A. T. Lewandowski, G. F. Payne, R. Ghodssi, W. E. Bentley and G. W. Rubloff, “Reversible biofunctionalization and catalytic activity of a metabolic pathway enzyme in reusable bioMEMS devices”, American Vacuum Society 54th International Symposium, Seattle, WA, October 14-19, 2007. (Oral presentation)
  24. X. L. Luo, J. J. Park, H. Yi, R. Ghodssi and G. W. Rubloff, “Biomolecule assembly and functionality in completely packaged microfluidic devices”, American Vacuum Society 53rd International Symposium, San Francisco, CA, November 12-17, 2006. (Oral presentation, 3rd Place Student Research Award)

Invited Seminars

  1. "Biofabrication-enabled synthetic ecosystems and static chemical gradients in microfluidics", ADA Foundation, Gaithersburg, MD, January 21, 2016.
  2. “Bacterial cell-cell signaling and chemotaxis enabled with biofabrication in microfluidics”, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, China, July 25, 2015.
  3. “Biofabrication in microfluidics to explore bacterial cell-cell signaling”, Department of Biology Seminar, Catholic University of America,  Washington DC, February 24, 2014.
  4. “Biofabrication in microfluidics to explore bacterial cell-cell signaling”, Mechanical Engineering Seminar, Catholic University of America,  Washington DC, November 29, 2012.
  5. “Biofabrication in microsystems to explore bacterial cell-cell signaling”, Plasma Physics Division Seminar, Naval Research Laboratory,  Washington DC, Sept. 7, 2012.
  6. “Biofabrication in microsystems to explore bacterial cell-cell signaling”, Mechanical Engineering Seminar, Texas Tech University, Lubbock, TX, Aug. 1, 2012.
  7. “Biofabrication: Interfacing biology with microfluidics”, Biomedical Engineering Seminar Series, Rutgers University, Piscataway, NJ, Mar. 7, 2011.
  8. “Biofabrication: Interfacing biology with microfluidics”, Mechanical Engineering Research Seminar, McGill University, Montreal, QC Canada, Feb. 17, 2011.
  9. “Biofabrication with Chitosan in Microfluidics for Interrogation of Quorum Sensing”, Novartis Institutes for BioMedical Research (NIBR), Boston, MA, Feb. 16, 2010.